SDS PAGE

sodium dodecyl sulphate - poly acrylamide gel electrophoresis

SDS PAGE

sodium dodecyl sulphate - poly acrylamide gel electrophoresis

Restriction endonucleases cut at different nucleotide in the same sequence.

Restriction endonuclease

Restriction endonucleases are the enzymes which cut(digest) DNA at specific sequence.

what is vector?

Vector is like vehicle by which we can make multiple copies of our gene and can express in the host.

Role of Alkaline phosphatase

Alkaline phosphatase provides basic condition to the medium . So that vector can not self ligate and our gene of interest ( gene which we want to insert in vector ) easily ligate with vector

Difference between RNA & DNA

DNA is most probably double stranded where as RNA is single stranded. DNA is having deoxyribose sugar whereas RNA is having ribose sugar.