restriction endonuclease

Restriction endonuclease is a group of enzymes which is use to cut the DNA at specific locations.
Depending upon the location different types of enzymes are used.
Such as type I restriction endonuclease
type II restriction endonuclease

hydrophilic

polar, water lover

Hydrophobic

non-polar, water hating

Role of calcium chloride in transformation

last date for csir-net for december 2015

is 25-08-2015

examples of type I restriction endonuclease

EcoB, EcoK

examples of type I restriction endonuclease

EcoB, EcoK

movement and requirements of type I restriction endonuclease

type I restriction endonucleases move along DNA in reaction and require Mg++, SAM (S-adenosyl methionine) and ATP as co-factor.

type I restriction endonuclease

Type I restriction endonucleases consist of three different subunits: 1. restrictive subunit which cut the DNA 2. modification subunit 3. specificity subunit which specifies the recognition site. These enzymes recognize the restriction site but they do not cut at the cleavage site or restriction site. These enzymes cut the DNA within the 1000bp away from recognition site or restriction site.

type I restriction endonuclease

Type I restriction endonucleases consist of three different subunits: 1. restrictive subunit which cut the DNA 2. modification subunit 3. specificity subunit which specifies the recognition site. These enzymes recognize the restriction site but they do not cut at the cleavage site or restriction site. These enzymes cut the DNA within the 15bp long sequence from recognition site or restriction site.

types of restriction endonucleases

there are three types of restriction endonucleases Type I Type II Type III Type II restriction endonucleases are the most preferable enzymes to cut the DNA in rDNA technology because of its specificity

Star activity of restriction endonuclease

restriction endonuclease cut at different position in the same sequence (restriction site).

vector must contain

1. ori region - origin of replication according to host. 2. selectable markers genes such ampicillin resistant gene, kanamycin resistant gene or tetracyclin resistant gene etc. 3. there should be MCS ( multiple cloning site) 4. there should be specific control systems such as promoter, terminator and RBS (ribosome binding site) etc

Role of SDS

SDS is a detergent. it can be use to rupture the cell wall and to degrade(breakdown in small fragments or mono units) the protein

SDS PAGE

sodium dodecyl sulphate - poly acrylamide gel electrophoresis

SDS PAGE

sodium dodecyl sulphate - poly acrylamide gel electrophoresis

Restriction endonucleases cut at different nucleotide in the same sequence.

Restriction endonuclease

Restriction endonucleases are the enzymes which cut(digest) DNA at specific sequence.

what is vector?

Vector is like vehicle by which we can make multiple copies of our gene and can express in the host.

Role of Alkaline phosphatase

Alkaline phosphatase provides basic condition to the medium . So that vector can not self ligate and our gene of interest ( gene which we want to insert in vector ) easily ligate with vector

Difference between RNA & DNA

DNA is most probably double stranded where as RNA is single stranded. DNA is having deoxyribose sugar whereas RNA is having ribose sugar.

Proteomics is the study of proteins.

proteome is the total amount of protein present in the cell.

Genomics is the study of gene.

if you have any doubt about biotechnology subject of any level ( 12, U.G. or P.G.)

The whole genetic material of a cell is called as "genome".

The whole genetic material of a cell is called as "genome".